B cell transcriptomes during EBV’s pre-latent phase
This page is an entry point to the rich biology of Epstein-Barr virus (EBV) and its preferred host cell. Here you will find cellular and viral genes and their regulation during the early time points after infection. We analyzed the transcriptomes of naïve B-lymphocytes from three different human donors at different stages.
You will be able to search for individual cellular or viral genes (“.t01”) and their expression levels during the pre-latent phase of viral infection from three donors at depicted time-points (day1, day2, day3, day4, day5, day8) and compare them with established, latently infected cells (day14) or B-lymphocytes prior to infection (day0). The gene list comprises all 55,955 cellular genes listed in the hg19 reference transcriptome as well as 50 EBV genes.
You can also look at and download six clusters of cellular genes. The clusters were identified and based on 11,860 cellular and viral genes that were recognized to be significantly regulated in the course of B-cell infection. After hierarchical clustering of these genes the clusters were named with an arbitrary color code. Each cluster combines genes with similar expression pattern identified in B cells during the pre-latent phase of EBV infection.
The gene list also contains entries that relate to the genetic composition of the recombinant wt/B95-8 EBV strain 2089 (Delecluse et al., 1998, Proc Natl Acad Sci U S A 95:8245–8250. doi: 10.1073/pnas.95.14.8245 ). Protein_A, Protein_B: The two genes refer to prokaryotic genes encoded by the mini-F factor backbone of the recombinant EBV strain. These genes are needed for DNA replication and partitioning of the plasmid in E.coli, only. They are under the control of prokaryotic promoters. Hygromycin, GFP: The genes are also located on the mini-F factor backbone and are ectopically expressed from the early promoter of SV40 and the immediate-early promoter of the human cytomegalovirus, respectively.
Cluster Average Expression
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt(GmbH)
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Dr. Antonio Scialdone